Tube-gel digestion: a novel proteomic approach for high throughput analysis of membrane proteins.

ABSTRACT: This study describes a new protein digestion protocol in which a variety of detergents can be used to solubilize membrane proteins and facilitate trypsin digestion with higher efficiency. In this protocol, proteins are dissolved in solutions containing various detergents and directly incorporated into a polyacrylamide gel matrix without electrophoresis. Detergents are subsequently eliminated from the gel matrix while proteins are still immobilized in the gel matrix. After in-gel digestion of proteins, LC-MS/MS is used to analyze the extracted peptides for protein identification. The uniqueness of the protocol is that it allows usage of a variety of detergents in the starting solution without interfering with LC-MS/MS analysis. We hereby demonstrate that different detergents, including ioni

SUBMITTER: Lu X 

PROVIDER: S-EPMC1360194 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature