Expression microarray and mouse virulence analysis of four conserved two-component gene regulatory systems in group a streptococcus.

ABSTRACT: Group A streptococcus (GAS) is a gram-positive human bacterial pathogen that causes diseases ranging from relatively mild epithelial cell surface infections to life-threatening invasive episodes. Much is known about the extracellular molecules that contribute to host-pathogen interactions, but in contrast, far less information is available about regulatory genes that control the expression of individual or multiple GAS virulence factors. The eight GAS genomes that have been sequenced have 12 conserved two-component gene regulatory systems (TCSs), but only 3 of these 12 have been studied in detail. Using an allelic replacement strategy with a nonpolar cassette, we inactivated the response regulator of four TCSs that have only weak homology with TCS genes of known or inferred function in oth

SUBMITTER: Sitkiewicz I 

PROVIDER: S-EPMC1360370 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature