Global analysis of Helicobacter pylori gene expression in human gastric mucosa.

ABSTRACT:

Background & aims

Helicobacter pylori inhabits a highly restricted ecological niche in the human gastric mucosa. Microbial gene expression in the context of persistent infection remains largely uncharacterized.

Methods

An RNA analysis method, selective capture of transcribed sequences, was used in conjunction with genomic array hybridization to characterize H. pylori complementary DNAs (cDNAs) obtained from both human and experimentally infected gerbil gastric tissue specimens.

Results

Bacterial cDNAs obtained by selective capture of transcribed sequences from tissues hybridized to arrayed DNA fragments representing approximately 70% of open reading frames in the H. pylori genome. RNAs for most of these open reading frames were also detected by array hybridization ana

SUBMITTER: Graham JE 

PROVIDER: S-EPMC1361305 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature