Multiomics profiling of bone in the Dpp3-deficient mice unveils altered protein and lipid composition.

ABSTRACT:

Purpose

Bone is a unique specialized connective tissue comprising inorganic and organic components which continuously undergoes remodeling through the activity of anabolic and catabolic pathways. Peptidases are enzymes cleaving peptide bonds in a broad range of substrates and hence involved in many pathophysiological mechanisms. We previously reported that lack of Dpp3 in the mouse model results in bone loss and here we aimed to establish whether it also affected bone matrix composition.

Methods

We performed proteomic and lipidomics analysis of the flushed bone of 6-month-old wild type (WT) and Dpp3 knock-out (ko) male mice (4 mice/genotype); Western blot analysis of selected upregulated proteins and gene expression analysis of genes potentially involved in the identified pa

SUBMITTER: Bevacqua E 

PROVIDER: S-EPMC13627175 | biostudies-literature | 2026 Oct

REPOSITORIES: biostudies-literature