Berberine targets the PI3Kα LYS672 site to suppress the PI3K/Akt/NF-κB axis and modulate microglial functional states after intracerebral hemorrhage.

ABSTRACT: Microglia-driven neuroinflammation contributes to secondary injury after intracerebral hemorrhage (ICH). Modulating microglial functional states represents a promising therapeutic strategy. Berberine (BBR) has neuroprotective effects, but its role in ICH remains unclear. This study investigated whether BBR alleviates ICH-induced neuroinflammation by modulating microglial responses after ICH via the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/nuclear factor kappa-B (NF-κB) axis. Network pharmacology predicted BBR targets. An ICH mouse model was used to assess BBR's effects on hematoma volume, neurological function and neuroinflammation. Transcriptomics identified differentially expressed genes and enriched pathways. Immunofluorescence assessed glial activation, cell death an

SUBMITTER: Wang Q 

PROVIDER: S-EPMC13634748 | biostudies-literature | 2026 Sep

REPOSITORIES: biostudies-literature