Peripheral human mAb therapy yields modulation of neuroinflammation and long-term functional recovery from lyssavirus infection.

ABSTRACT: Rabies is a fatal encephalitis caused by viruses in the lyssavirus genus. We previously demonstrated that intraperitoneal administration of a single dose of neutralizing human monoclonal antibody (mAb) F11 can protect mice from fatal lyssavirus infection, post-central nervous system (CNS) invasion. However, the molecular basis of F11 neutralization remains unknown. Here, we use structural and functional analyses to define neutralizing activity of F11 and the related mAb, A6. Cryo-electron microscopy (EM) and negative-stain EM reveal that both mAbs bind Domain III of the glycoprotein G, recognizing a prefusion-specific epitope distinct from previously characterized antibodies including RVC20. Binding stabilizes the prefusion state, blocking membrane fusion. We furthermore found that a singl

SUBMITTER: Huaman C 

PROVIDER: S-EPMC13637774 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature