Detecting hidden threats: proteomic changes in urinary extracellular vesicles are associated with pediatric kidney allograft rejection.

ABSTRACT:

Background

Early detection of kidney allograft injury is essential to preserve long-term graft function. Current diagnostics, particularly biopsy, are invasive and may be inconclusive. Extracellular-vesicles (EVs) carry molecular cargo that reflects renal cellular states. Although EV proteomics has shown promise for detecting acute rejection (AR) in adults, its value in pediatrics(PKT) is not well defined.

Methods

In this pilot study, urine samples from 29 PKT recipients and 3 healthy controls were analyzed, including 7 with biopsy-confirmed AR, 9 with chronic rejection (CR), and 13 with stable graft function (ST). Classification was based on urine findings, creatinine trends, and biopsy results. EVs were isolated from fresh-second-void urine by ultracentrifugation and analy

SUBMITTER: Daniella LE 

PROVIDER: S-EPMC13638442 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature