Engineered outer membrane vesicle-like nanoparticles from Lactobacillus plantarum for oral IL-22 mRNA delivery and treatment of DSS-induced colitis.

ABSTRACT: This study developed an engineered Lactobacillus plantarum-derived vesicle system for oral delivery of interleukin-22 mRNA (IL-22 mRNA) and evaluated its effects on epithelial barrier repair in DSS-induced colitis. The vesicle formulation, termed P-OMVs@IL-22 m, was prepared from L. plantarum protoplasts through lysozyme treatment, ultrasonic disruption, OptiPrep density-gradient purification, and incubation-based mRNA loading. The formulation achieved an IL-22 mRNA encapsulation efficiency of 72.43 ± 6.60% and a loading capacity of 35.97 ± 3.67 ng mRNA/µg P-OMV protein. mRNA loading increased the mean particle diameter from 119 ± 3 to 389 ± 7 nm and reduced the detectable particle concentration from 7.83 ± 0.81 × 10⁹ to 6.00 ± 0.78 × 10⁹ particles/mL. P-OMVs@IL-22 m protected IL-22 mRNA f

SUBMITTER: Yuan W 

PROVIDER: S-EPMC13638770 | biostudies-literature | 2026 Aug

REPOSITORIES: biostudies-literature