An advanced genome engineering platform for Komagataella phaffii enabling versatile in vivo DNA assembly and multiplex integration.

ABSTRACT:

Background

The transition toward a sustainable bioeconomy necessitates the development of robust microbial platforms capable of C1 chemical valorization, particularly the conversion of methanol into high-value chemicals. Komagataella phaffii is a premier methylotrophic host for such applications due to its natural methanol utilization pathways and high-density fermentation capabilities. However, its potential as a C1-based synthetic biology chassis has been hampered by inefficient homologous recombination and the technical complexity of multigene pathway integration. To accelerate the design-build-test-learn (DBTL) cycle for methanol-based biomanufacturing, a more sophisticated and high-throughput genome engineering toolkit is required.

Results

We report the development of a

SUBMITTER: Choi Y 

PROVIDER: S-EPMC13640322 | biostudies-literature | 2026 Jul

REPOSITORIES: biostudies-literature