A biomimetic cryoprotectant preserves extracellular vesicles bioactivity for intravenous administration and potentiates wound healing.

ABSTRACT: Extracellular vesicles (EVs) have emerged as promising vehicles for drug delivery and disease therapy owing to their intrinsic biocompatibility and capacity to transfer functional biomolecules. However, limitations in current storage technologies, including structural degradation and bioactivity loss during long-term freezing, and the incompatibility of conventional cryoprotectants such as DMSO and glycerol with intravenous administration, pose major barriers to clinical translation. Herein, we developed DDAS, a biomimetic cryoprotectant formulation, rationally designed through generative artificial intelligence (GAI)-assisted prioritization of physiologically relevant candidates from the Human Metabolome Database. DDAS comprises dextran, albumin, and sialic acid, which were selected for t

SUBMITTER: Zhang P 

PROVIDER: S-EPMC13644063 | biostudies-literature | 2026 Jul

REPOSITORIES: biostudies-literature