High-efficiency 14-plex cSNP SNaPshot assay for forensic peripheral blood identification.

ABSTRACT: The analysis of body fluid stains found at criminal scenes requires not only DNA profiles but also identification of the composition of these stains, particularly in mixed stains. Prior studies have demonstrated that forensic body fluids can be differentiated by their mRNA biomarkers.In this study, we screened a panel including 14 cSNPs located on four peripheral blood mRNA biomarkers (CD3G, ANK1, SPTB, and GYPA) and developed a multiplex assay for forensic blood identification using the SNaPshot method. Particularly, a one-step multiplex reverse transcription PCR (RT-PCR) strategy was used to generate cDNA amplicons from the corresponding mRNA by reverse transcription. The sensitivity, specificity, and capability of the multiplex SNaPshot assay for blood identification were systematically

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC13644189 | biostudies-literature | 2026 Jul

REPOSITORIES: biostudies-literature