Remodeling of cortical bone allografts mediated by adherent rAAV-RANKL and VEGF gene therapy.

ABSTRACT: Structural allograft healing is limited because of a lack of vascularization and remodeling. To study this we developed a mouse model that recapitulates the clinical aspects of live autograft and processed allograft healing. Gene expression analyses showed that there is a substantial decrease in the genes encoding RANKL and VEGF during allograft healing. Loss-of-function studies showed that both factors are required for autograft healing. To determine whether addition of these signals could stimulate allograft vascularization and remodeling, we developed a new approach in which rAAV can be freeze-dried onto the cortical surface without losing infectivity. We show that combination rAAV-RANKL- and rAAV-VEGF-coated allografts show marked remodeling and vascularization, which leads to a new bo

SUBMITTER: Ito H 

PROVIDER: S-EPMC1364464 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature