KIF14 silencing suppresses cell proliferation and enhances chemosensitivity by modulating 53BP1 nucleocytoplasmic shuttling in non-WNT/non-SHH medulloblastoma.

ABSTRACT: Medulloblastoma (MB) is the most common malignant pediatric brain tumor. The non-WNT/non-SHH subtypes (Groups 3 and 4) exhibit high genetic heterogeneity and limited therapeutic options. Kinesin family member 14 (KIF14) is selectively expressed during nervous system development. However, its role in non-WNT/non-SHH MB remains poorly understood. In this study, analysis of the GEO database revealed that high KIF14 expression correlates with poor prognosis in patients with non-WNT/non-SHH MB. Silencing KIF14 in MB cells impaired cytokinesis, leading to p53-mediated cell cycle arrest and apoptosis. Mechanistically, KIF14 knockdown disrupted the nucleocytoplasmic shuttling of the p53-binding protein 53BP1, resulting in its nuclear accumulation. This shift inhibited homologous recombination (HR)

SUBMITTER: Fang H 

PROVIDER: S-EPMC13645811 | biostudies-literature | 2026 Jul

REPOSITORIES: biostudies-literature