Repeatable, low-drift recordings in behaving nonhuman primates using flexible microelectrodes.

ABSTRACT: Neurophysiological recordings from nonhuman primates rely on rigid microelectrode arrays whose mechanical mismatch with brain tissue causes neuronal drift and unstable single-unit recordings. Here we present a flexible microelectrode platform for repeated recordings in awake rhesus macaques. We fabricated a 32-channel, 7-μm-thick Parylene-C array and developed a telescopic insertion method using concentric guide tubes and a retractable microwire shuttle to repeatedly deliver free-floating arrays through intact dura using standard recording chambers. Across two animals, we optimized the electrode geometry and insertion to achieve an 80% single-unit recording success rate. During an oculomotor delayed response task, we recorded stable task-responsive neurons from prefrontal and posterior par

SUBMITTER: Woods DP 

PROVIDER: S-EPMC13649665 | biostudies-literature | 2026 Sep

REPOSITORIES: biostudies-literature