Accuracy and dynamic range of spatial image correlation and cross-correlation spectroscopy.

ABSTRACT: We present a comprehensive study of the accuracy and dynamic range of spatial image correlation spectroscopy (ICS) and image cross-correlation spectroscopy (ICCS). We use simulations to model laser scanning microscopy imaging of static subdiffraction limit fluorescent proteins or protein clusters in a cell membrane. The simulation programs allow us to control the spatial imaging sampling variables and the particle population densities and interactions and introduce and vary background and counting noise typical of what is encountered in digital optical microscopy. We systematically calculate how the accuracy of both image correlation methods depends on practical experimental collection parameters and characteristics of the sample. The results of this study provide a guide to appropriately

SUBMITTER: Costantino S 

PROVIDER: S-EPMC1366609 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature