Variable incidence angle fluorescence interference contrast microscopy for z-imaging single objects.

ABSTRACT: Surface-generated structured illumination microscopies interrogate the position of fluorescently labeled objects near surfaces with nanometer resolution along the z axis. However, these techniques are either experimentally cumbersome or applicable to a limited set of experimental systems. We present a new type of surface-generated structured illumination fluorescence microscopy, variable incidence angle fluorescence interference contrast microscopy (VIA-FLIC), in which the fluorescent sample is assembled above a reflective Si surface and the incidence angle of excitation light is varied by placing annular photomasks with different radii in the aperture diaphragm plane of the microscope. The variation in incidence angle alters the interference pattern of excitation light, and hence the inte

SUBMITTER: Ajo-Franklin CM 

PROVIDER: S-EPMC1366776 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature