Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis.

ABSTRACT: Fluorescence lifetime imaging microscopy presents a powerful tool in biology and biophysics because it allows the investigation of the local environment of a fluorochrome in living cells in a quantitative manner. Furthermore, imaging Förster-type resonance energy transfer (FRET) by fluorescence lifetime imaging microscopy enables protein-protein interactions and intermolecular distances to be mapped under physiological conditions. Quantitative and precise data analysis methods are required to access the richness of information that is contained in FRET data on biological samples. Lifetime detection in the frequency-domain yields two lifetime estimations. The lifetime moments analysis (LiMA) provides a quantitative measure of the lifetime distribution broadness by exploiting the analytical

SUBMITTER: Esposito A 

PROVIDER: S-EPMC1366993 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature