A two-photon excitation fluorescence cross-correlation assay for a model ligand-receptor binding system using quantum dots.

ABSTRACT: Two-photon excitation fluorescence cross-correlation spectroscopy (TPE-XCS) is a very suitable method for studying interactions of two distinctly labeled fluorescent molecules. As such, it lends itself nicely to the study of ligand-receptor interactions. By labeling the ligand with one color of fluorescent dye and the receptor with another, it is possible to directly monitor ligand binding rather than inferring binding by monitoring downstream effects. One challenge of the TPE-XCS approach is that of separating the signal due to the receptor from that of the ligand. Using standard organic fluorescent labels there is almost inevitably spectral cross talk between the detection channels, which must be accounted for in TPE-XCS data analysis. However, using quantum dots as labels for both ligan

SUBMITTER: Swift JL 

PROVIDER: S-EPMC1367290 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature