Genome-scale thermodynamic analysis of Escherichia coli metabolism.

ABSTRACT: Genome-scale metabolic models are an invaluable tool for analyzing metabolic systems as they provide a more complete picture of the processes of metabolism. We have constructed a genome-scale metabolic model of Escherichia coli based on the iJR904 model developed by the Palsson Laboratory at the University of California at San Diego. Group contribution methods were utilized to estimate the standard Gibbs free energy change of every reaction in the constructed model. Reactions in the model were classified based on the activity of the reactions during optimal growth on glucose in aerobic media. The most thermodynamically unfavorable reactions involved in the production of biomass in E. coli were identified as ATP phosphoribosyltransferase, ATP synthase, methylene-tetra-hydrofolate dehydrogen

SUBMITTER: Henry CS 

PROVIDER: S-EPMC1367295 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature