SW-620 cells treated with topoisomerase I inhibitor SN-38: gene expression profiling.

ABSTRACT:

Background

The goal of this study was to evaluate changes in gene expression in SW-620 cells in response to SN-38 in order to further elucidate the mechanisms by which SN-38 causes apoptosis and cell cycle arrest.

Methods

We used a quantitative gene expression microarray assay to identify the genes regulated by SN-38 treatment in colon cancer cells and confirmed our results with RT-PCR. By gene expression profiling, we first screened a proprietary list of about 22,000 genes.

Results

Treatment with SN-38 cells resulted in two-fold or greater alteration in the level of expression of 192 genes compared to control treatment. Most of the affected genes were not known to be responsive to SN-38 prior to this study. SN-38 treatment of these cells was found to affect the expre

SUBMITTER: Souza V 

PROVIDER: S-EPMC1368997 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature