Construction and validation of the APOCHIP, a spotted oligo-microarray for the study of beta-cell apoptosis.

ABSTRACT:

Background

Type 1 diabetes mellitus (T1DM) is a autoimmune disease caused by a long-term negative balance between immune-mediated beta-cell damage and beta-cell repair/regeneration. Following immune-mediated damage the beta-cell fate depends on several genes up- or down-regulated in parallel and/or sequentially. Based on the information obtained by the analysis of several microarray experiments of beta-cells exposed to pro-apoptotic conditions (e.g. double stranded RNA (dsRNA) and cytokines), we have developed a spotted rat oligonucleotide microarray, the APOCHIP, containing 60-mer probes for 574 genes selected for the study of beta-cell apoptosis.

Results

The APOCHIP was validated by a combination of approaches. First we performed an internal validation of the spotted probe

SUBMITTER: Magnusson NE 

PROVIDER: S-EPMC1368999 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature