High-throughput trapping of secretory pathway genes in mouse embryonic stem cells.

ABSTRACT: High-throughput gene trapping is a random approach for inducing insertional mutations across the mouse genome. This approach uses gene trap vectors that simultaneously inactivate and report the expression of the trapped gene at the insertion site, and provide a DNA tag for the rapid identification of the disrupted gene. Gene trapping has been used by both public and private institutions to produce libraries of embryonic stem (ES) cells harboring mutations in single genes. Presently, approximately 66% of the protein coding genes in the mouse genome have been disrupted by gene trap insertions. Among these, however, genes encoding signal peptides or transmembrane domains (secretory genes) are underrepresented because they are not susceptible to conventional trapping methods. Here, we describe

SUBMITTER: De-Zolt S 

PROVIDER: S-EPMC1369290 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature