Perturbation of transcription elongation influences the fidelity of internal exon inclusion in Saccharomyces cerevisiae.

ABSTRACT: Unknown mechanisms exist to ensure that exons are not skipped during biogenesis of mRNA. Studies have connected transcription elongation with regulated alternative exon inclusion. To determine whether the relative rates of transcription elongation and spliceosome assembly might play a general role in enforcing constitutive exon inclusion, we measured exon skipping for a natural two-intron gene in which the internal exon is constitutively included in the mRNA. Mutations in this gene that subtly reduce recognition of the intron 1 branchpoint cause exon skipping, indicating that rapid recognition of the first intron is important for enforcing exon inclusion. To test the role of transcription elongation, we treated cells to increase or decrease the rate of transcription elongation. Consistent

SUBMITTER: Howe KJ 

PROVIDER: S-EPMC1370465 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature