Chimeric RNA transposition intermediates of the I factor produce precise retrotransposed copies.

ABSTRACT: I elements in Drosophila melanogaster are non-long terminal repeat (LTR) retrotransposons of particular interest because high levels of transposition can be induced by appropriate crosses. They use a full-length RNA transposition intermediate as a template for reverse transcription. Detailed molecular characterization of this intermediate is rendered difficult because of the many transcripts produced by defective elements. The use of an active I element marked with a sequence encoding the HA epitope solves this problem. We used an RNA circularization procedure followed by RT-PCR to analyze the transcripts produced by actively transposing tagged I elements. Most start at the 5' end at the second nucleotide of the I element and all are polyadenylated at a site located in genomic sequences do

SUBMITTER: Chambeyron S 

PROVIDER: S-EPMC137080 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature