An in vitro RNA editing system from cauliflower mitochondria: editing site recognition parameters can vary in different plant species.

ABSTRACT: Most of the 400 RNA editing sites in flowering plant mitochondria are found in mRNAs. Consequently, the sequence vicinities of homologous sites are highly conserved between different species and are presumably recognized by likewise conserved trans-factors. To investigate the evolutionary adaptation to sequence variation, we have now analyzed the recognition elements of an editing site with divergent upstream sequences in the two species pea and cauliflower. This variation is tolerated at the site selected, because the upstream cis-elements reach into the 5'-UTR of the mRNA. To compare cis-recognition features in pea and cauliflower mitochondria, we developed a new in vitro RNA editing system for cauliflower. In vitro editing assays with deleted and mutated template RNAs show that the majo

SUBMITTER: Neuwirt J 

PROVIDER: S-EPMC1370840 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature