Biotin-tagged cDNA expression libraries displayed on lambda phage: a new tool for the selection of natural protein ligands.

ABSTRACT: cDNA expression libraries displayed on lambda phage have been successfully employed to identify partners involved in antibody-antigen, protein- protein and DNA-protein interactions and represent a novel approach to functional genomics. However, as in all other cDNA expression libraries based on fusion to a carrier polypeptide, a major issue of this system is the absence of control over the translation frame of the cDNA. As a consequence, a large number of clones will contain lambda D/cDNA fusions, resulting in the foreign sequence being translated on alternative reading frames. Thus, many phage will not display natural proteins, but could be selected, as they mimic the binding properties of the real ligand, and will hence interfere with the selection outcome. Here we describe a novel lambd

SUBMITTER: Ansuini H 

PROVIDER: S-EPMC137096 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature