Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer.

ABSTRACT: Reagents for proteome research must of necessity be generated by high throughput methods. Aptamers are potentially useful as reagents to identify and quantitate individual proteins, yet are currently produced for the most part by manual selection procedures. We have developed automated selection methods, but must still individually purify protein targets. Therefore, we have attempted to select aptamers against protein targets generated by in vitro transcription and translation of individual genes. In order to specifically immobilize the protein targets for selection, they are also biotinylated in vitro. As a proof of this method, we have selected aptamers against translated human U1A, a component of the nuclear spliceosome. Selected sequences demonstrated exquisite mimicry of natural bindi

SUBMITTER: Cox JC 

PROVIDER: S-EPMC137152 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature