Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export.

ABSTRACT:

Background

Metabolic pathway manipulation for improving the properties and the productivity of microorganisms is becoming a well established concept. For the production of important metabolites, but also for a better understanding of the fundamentals of cell biology, detailed studies are required. In this work we analysed the lactate production from metabolic engineered Saccharomyces cerevisiae cells expressing a heterologous lactate dehydrogenase (LDH) gene. The LDH gene expression in a budding yeast cell introduces a novel and alternative pathway for the NAD+ regeneration, allowing a direct reduction of the intracellular pyruvate to lactate, leading to a simultaneous accumulation of lactate and ethanol.

Results

Four different S. cerevisiae strains were transformed with six

SUBMITTER: Branduardi P 

PROVIDER: S-EPMC1373645 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature