Development of a new bicistronic retroviral vector with strong IRES activity.

ABSTRACT:

Background

Internal Ribosome Entry Site (IRES)-based bicistronic vectors are important tools in today's cell biology. Among applications, the expression of two proteins under the control of a unique promoter permits the monitoring of expression of a protein whose biological function is being investigated through the observation of an easily detectable tracer, such as Green Fluorescent Protein (GFP). However, analysis of published results making use of bicistronic vectors indicates that the efficiency of the IRES-controlled expression can vary widely from one vector to another, despite their apparent identical IRES sequences. We investigated the molecular basis for these discrepancies.

Results

We observed up to a 10 fold difference in IRES-controlled expression from distinct

SUBMITTER: Martin P 

PROVIDER: S-EPMC1373653 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature