Sequence specific visual detection of LAMP reactions by addition of cationic polymers.

ABSTRACT:

Background

Development of a practical gene point-of-care testing device (g-POCT device) requires innovative detection methods for demonstrating the results of the gene amplification reaction without the use of expensive equipment. We have studied a new method for the sequence-specific visual detection of minute amounts of nucleic acids using precipitation reaction by addition of cationic polymers to amplicons of Loop mediated isothermal Amplification (LAMP).

Results

Oligo DNA probes labeled with different fluorescent dyes were prepared for multiple nucleic acid templates, and the templates were amplified by the LAMP reactions under the existence of the probes. At completion of the LAMP reaction, an optimal amount of low molecular weight polyethylenimine (PEI) was added, resu

SUBMITTER: Mori Y 

PROVIDER: S-EPMC1373654 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature