Recovery and evolutionary analysis of complete integron gene cassette arrays from Vibrio.

ABSTRACT:

Background

Integrons are genetic elements capable of the acquisition, rearrangement and expression of genes contained in gene cassettes. Gene cassettes generally consist of a promoterless gene associated with a recombination site known as a 59-base element (59-be). Multiple insertion events can lead to the assembly of large integron-associated cassette arrays. The most striking examples are found in Vibrio, where such cassette arrays are widespread and can range from 30 kb to 150 kb. Besides those found in completely sequenced genomes, no such array has yet been recovered in its entirety. We describe an approach to systematically isolate, sequence and annotate large integron gene cassette arrays from bacterial strains.

Results

The complete Vibrio sp. DAT722 integron cassette

SUBMITTER: Boucher Y 

PROVIDER: S-EPMC1382264 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature