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Potential for alternative intron-exon pairings in group II intron RmInt1 from Sinorhizobium meliloti and its relatives.

ABSTRACT: Ribozyme constructs derived from group II intron RmInt1 of Sinorhizobium meliloti self-splice in vitro when incubated under permissive conditions, but exon ligation is unusually inefficient when the 5' exon is truncated close to the IBS2 intron-binding site. One plausible explanation for this observation is the presence of an alternative intron-exon pairing between an intron segment that overlaps with the EBS2 exon-binding site and a 5' exon site located just distal of IBS2 relative to the splice junction. Strikingly, the existence of this pairing is supported by comparative sequence analysis of introns related to RmInt1.

SUBMITTER: Costa M 

PROVIDER: S-EPMC1383573 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature

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Potential for alternative intron-exon pairings in group II intron RmInt1 from Sinorhizobium meliloti and its relatives.

Costa María M   Michel François F   Toro Nicolás N  

RNA (New York, N.Y.) 20060123 3

Ribozyme constructs derived from group II intron RmInt1 of Sinorhizobium meliloti self-splice in vitro when incubated under permissive conditions, but exon ligation is unusually inefficient when the 5' exon is truncated close to the IBS2 intron-binding site. One plausible explanation for this observation is the presence of an alternative intron-exon pairing between an intron segment that overlaps with the EBS2 exon-binding site and a 5' exon site located just distal of IBS2 relative to the splic  ...[more]

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