Phosphorylation-facilitated sumoylation of MEF2C negatively regulates its transcriptional activity.

ABSTRACT:

Background

Sumoylation has emerged as an important posttranslational regulatory mechanism for transcription factors and cofactors. Sumoylation of many transcription factors represses their transcriptional activities. The myocyte enhancer factor 2 (MEF2) family of transcription factors plays an important role in regulating gene expression during myogenesis and has been recently shown to be sumoylated.

Results

Consistent with earlier reports, we show that sumoylation of MEF2C at K391 inhibits its transcriptional activity. Sumoylation of MEF2C does not block its DNA-binding activity. A small C-terminal fragment of MEF2C containing K391, referred to as delta-N2-MEF2C, is efficiently sumoylated and, when targeted to DNA, represses transcription at neighbouring promoters. Because

SUBMITTER: Kang J 

PROVIDER: S-EPMC1386686 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature