Germ cell development in the Honeybee (Apis mellifera); vasa and nanos expression.

ABSTRACT:

Background

Studies of specification of germ-cells in insect embryos has indicated that in many taxa the germ cells form early in development, and their formation is associated with pole plasm, germ plasm or an organelle called the oosome. None of these morphological features associated with germ cell formation have been identified in the Honeybee Apis mellifera. In this study I report the cloning and expression analysis of Honeybee homologues of vasa and nanos, germ cell markers in insects and other animals.

Results

Apis vasa and nanos RNAs are present in early honeybee embryos, but the RNAs clear rapidly, without any cells expressing these germ cell markers past stage 2. These genes are then only expressed in a line of cells in the abdomen from stage 9 onwards. These cells

SUBMITTER: Dearden PK 

PROVIDER: S-EPMC1388196 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature