Antigenomic delta ribozyme variants with mutations in the catalytic core obtained by the in vitro selection method.

ABSTRACT: We have used the in vitro selection method to search for catalytically active variants of the antigenomic delta ribozyme with mutations in the regions that constitute the ribozyme active site: L3, J1/4 and J4/2. In the initial combinatorial library 16 nt positions were randomized and the library contained a full representation of all possible sequences. Following ten cycles of selection-amplification several catalytically active ribozyme variants were identified. It turned out that one-third of the variants contained only single mutation G80U and their activity was similar to that of the wild-type ribozyme. Unexpectedly, in the next one-third of the variants the C76 residue, which was proposed to play a crucial role in the ribozyme cleavage mechanism, was mutated. In these variants, howeve

SUBMITTER: Legiewicz M 

PROVIDER: S-EPMC1388270 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature