Global identification of noncoding RNAs in Saccharomyces cerevisiae by modulating an essential RNA processing pathway.

ABSTRACT: Noncoding RNAs (ncRNAs) perform essential cellular tasks and play key regulatory roles in all organisms. Although several new ncRNAs in yeast were recently discovered by individual studies, to our knowledge no comprehensive empirical search has been conducted. We demonstrate a powerful and versatile method for global identification of previously undescribed ncRNAs by modulating an essential RNA processing pathway through the depletion of a key ribonucleoprotein enzyme component, and monitoring differential transcriptional activities with genome tiling arrays during the time course of the ribonucleoprotein depletion. The entire Saccharomyces cerevisiae genome was scanned during cell growth decay regulated by promoter-mediated depletion of Rpp1, an essential and functionally conserved protei

SUBMITTER: Samanta MP 

PROVIDER: S-EPMC1389707 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature