Sequencing of hsp65 distinguishes among subsets of the Mycobacterium avium complex.

ABSTRACT: The Mycobacterium avium complex consists of epidemiologically distinct subsets. The classification of these subsets is complicated by a number of factors, including the ambiguous results obtained with phenotypic and genetic assays and the recent appreciation that human and avian strains appear to be distinct. In previous work, sequencing based on a 441-bp portion of the hsp65 gene has proven to efficiently classify isolates within the Mycobacterium genus but provides low resolution for distinguishing among members of the M. avium complex. Therefore, in this study, we have targeted the more variable 3' region of the hsp65 gene to determine whether it can effectively discriminate M. avium complex isolates at the levels of species and subspecies. Primers designed for this target consistently

SUBMITTER: Turenne CY 

PROVIDER: S-EPMC1392684 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature