Characterization of the tre locus and analysis of trehalose cryoprotection in Lactobacillus acidophilus NCFM.

ABSTRACT: Freezing and lyophilization are common methods used for preservation and storage of microorganisms during the production of concentrated starter cultures destined for industrial fermentations or product formulations. The compatible solute trehalose has been widely reported to protect bacterial, yeast and animal cells against a variety of environmental stresses, particularly freezing and dehydration. Analysis of the Lactobacillus acidophilus NCFM genome revealed a putative trehalose utilization locus consisting of a transcriptional regulator, treR; a trehalose phosphoenolpyruvate transferase system (PTS) transporter, treB; and a trehalose-6-phosphate hydrolase, treC. The objective of this study was to characterize the tre locus in L. acidophilus and determine whether or not intracellular up

SUBMITTER: Duong T 

PROVIDER: S-EPMC1392980 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature