Genetic identification of the main opportunistic Mucorales by PCR-restriction fragment length polymorphism.

ABSTRACT: Mucormycosis is a rare and opportunistic infection caused by fungi belonging to the order Mucorales. Recent reports have demonstrated an increasing incidence of mucormycosis, which is frequently lethal, especially in patients suffering from severe underlying conditions such as immunodeficiency. In addition, even though conventional mycology and histopathology assays allow for the identification of Mucorales, they often fail in offering a species-specific diagnosis. Due to the lack of other laboratory tests, a precise identification of these molds is thus notoriously difficult. In this study we aimed to develop a molecular biology tool to identify the main Mucorales involved in human pathology. A PCR strategy selectively amplifies genomic DNA from molds belonging to the genera Absidia, Muco

SUBMITTER: Machouart M 

PROVIDER: S-EPMC1393117 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature