Single-nucleotide repeat analysis for subtyping Bacillus anthracis isolates.

ABSTRACT: Single-nucleotide repeats (SNRs) are variable-number tandem repeats that display very high mutation rates. In an outbreak situation, the use of a marker system that exploits regions with very high mutation rates, such as SNRs, allows the differentiation of isolates with extremely low levels of genetic diversity. This report describes the identification and analysis of SNR loci of Bacillus anthracis. SNR loci were selected in silico, and the loci with the highest diversity were used to design and test locus-specific primers against a number of B. anthracis strains with the same multilocus variable-number tandem repeat analysis (MLVA) genotype. SNR markers that allowed strains with the same MLVA genotype to be differentiated from each other were identified. The resulting SNR marker system ca

SUBMITTER: Stratilo CW 

PROVIDER: S-EPMC1393151 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature