High-throughput isolation of ultra-pure plasmid DNA by a robotic system.

ABSTRACT:

Background

With the availability of complete genomes, a systematic inventory of cellular processes becomes achievable. This requires assessing the function of all individual genes. Transfection of plasmid DNA into cell culture cells is an essential technique for this aim as it allows functional overexpression or downregulation of genes. While many robotic systems isolate plasmids for sequencing purposes, for more demanding applications such as transfections there is a shortage of robots for the high-throughput isolation of plasmid DNA.

Results

Here we describe a custom-made, automated device, which uses a special protocol to isolate plasmid DNAs with a purity sufficient for efficient transfections into mammalian cells. Approximately 1,600 ultra pure plasmids can be isolated

SUBMITTER: Kachel V 

PROVIDER: S-EPMC1395312 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature