A small loop in the capsid protein of Moloney murine leukemia virus controls assembly of spherical cores.

ABSTRACT: We report the identification of a novel domain in the Gag protein of Moloney murine leukemia virus (MoLV) that is important for the formation of spherical cores. Analysis of 18 insertional mutations in the N-terminal domain of the capsid protein (CA) identified 3 that were severely defective for viral assembly and release. Transmission electron microscopy of cells producing these mutants showed assembly of Gag proteins in large, flat or dome-shaped patches at the plasma membrane. Spherical cores were not formed, and viral particles were not released. This late assembly/release block was partially rescued by wild-type virus. All three mutations localized to the small loop between alpha-helices 4 and 5 of CA, analogous to the cyclophilin A-binding loop of human immunodeficiency virus type 1

SUBMITTER: Auerbach MR 

PROVIDER: S-EPMC1395457 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature