Alpha-Galactosidase Aga27A, an enzymatic component of the Clostridium josui cellulosome.

ABSTRACT: The Clostridium josui aga27A gene encodes the cellulosomal alpha-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various alpha-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A alpha-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose.

SUBMITTER: Jindou S 

PROVIDER: S-EPMC139563 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature