Simple microdilution test for detection of metallo-beta-lactamase production in Pseudomonas aeruginosa.

ABSTRACT: A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM- and 4 IMP-type metallo-beta-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.

SUBMITTER: Migliavacca R 

PROVIDER: S-EPMC139638 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature