Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection.

ABSTRACT:

Background

Enterobacter sakazakii is a foodborne pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. The current FDA detection method includes two enrichment steps, the subculturing of the second enrichment broth on a selective agar (VRBG), a further subculturing of selected grown colonies on TSA and the subsequent biochemical identification of yellow-pigmented colonies by API20E. However, there is a strong need for simplified methods for isolation and identification of E. sakazakii. In this study, two chromogenic media, which allow to indicate presumptive E. sakazakii colonies by the alpha glucosidase activity, as well as a newly developed 1,6-alpha-glucosidase based conventional PCR a

SUBMITTER: Lehner A 

PROVIDER: S-EPMC1397842 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature