Mutational analysis of the Saccharomyces cerevisiae cytochrome c oxidase assembly protein Cox11p.

ABSTRACT: Cox11p is an integral protein of the inner mitochondrial membrane that is essential for cytochrome c oxidase assembly. The bulk of the protein is located in the intermembrane space and displays high levels of evolutionary conservation. We have analyzed a collection of site-directed and random cox11 mutants in an effort to further define essential portions of the molecule. Of the alleles studied, more than half had no apparent effect on Cox11p function. Among the respiration deficiency-encoding alleles, we identified three distinct phenotypes, which included a set of mutants with a misassembled or partially assembled cytochrome oxidase, as indicated by a blue-shifted cytochrome aa(3) peak. In addition to the shifted spectral signal, these mutants also display a specific reduction in the lev

SUBMITTER: Banting GS 

PROVIDER: S-EPMC1398067 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature