Outliers involving the Poly(A) effect among highly-expressed genes in microarrays.

ABSTRACT:

Background

The Poly(A) effect is a cross-hybridization artifact in which poly(T)-containing molecules, which are produced by the reverse transcription of a poly(A)+ RNA mixture, bind promiscuously to the poly(A) stretches of the DNA in microarray spots. It is customary to attempt to block such hybridization by adding poly(A) to the hybridization solution. This note describes an experiment intended to evaluate circumstances under which the blocking procedure may not have been successful.

Results

The experiment involves a spot-by-spot comparison between the hybridization signals obtained by hybridizing a microarray to: (1) end-labeled oligo(dT), versus, (2) cDNA prepared from muscle tissue. We found that the blocking appears to be successful for the vast majority of microarray

SUBMITTER: Pan SJ 

PROVIDER: S-EPMC140022 | biostudies-literature | 2002 Dec

REPOSITORIES: biostudies-literature