Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.

ABSTRACT: Genome-wide identification of RNAs associated with RNA-binding proteins is crucial for deciphering posttranscriptional regulatory systems. PUMILIO is a member of the evolutionary conserved Puf-family of RNA-binding proteins that repress gene expression posttranscriptionally. We generated transgenic flies expressing affinity-tagged PUMILIO under the control of an ovary-specific promoter, and we purified PUMILIO from whole adult flies and embryos and analyzed associated mRNAs by using DNA microarrays. Distinct sets comprising hundreds of mRNAs were associated with PUMILIO at the two developmental stages. Many of these mRNAs encode functionally related proteins, supporting a model for coordinated regulation of posttranscriptional modules by specific RNA-binding proteins. We identified a chara

SUBMITTER: Gerber AP 

PROVIDER: S-EPMC1400586 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature