Cynomolgus monkey testicular cDNAs for discovery of novel human genes in the human genome sequence.

ABSTRACT:

Background

In order to contribute to the establishment of a complete map of transcribed regions of the human genome, we constructed a testicular cDNA library for the cynomolgus monkey, and attempted to find novel transcripts for identification of their human homologues.

Result

The full-insert sequences of 512 cDNA clones were determined. Ultimately we found 302 non-redundant cDNAs carrying open reading frames of 300 bp-length or longer. Among them, 89 cDNAs were found not to be annotated previously in the Ensembl human database. After searching against the Ensembl mouse database, we also found 69 putative coding sequences have no homologous cDNAs in the annotated human and mouse genome sequences in Ensembl. We subsequently designed a DNA microarray including 396 non-redundan

SUBMITTER: Osada N 

PROVIDER: S-EPMC140308 | biostudies-literature | 2002 Dec

REPOSITORIES: biostudies-literature