Engineered viruses to select genes encoding secreted and membrane-bound proteins in mammalian cells.

ABSTRACT: We have developed a functional genomics tool to identify the subset of cDNAs encoding secreted and membrane-bound proteins within a library (the 'secretome'). A Sindbis virus replicon was engineered such that the envelope protein precursor no longer enters the secretory pathway. cDNA fragments were fused to the mutant precursor and expression screened for their ability to restore membrane localization of envelope proteins. In this way, recombinant replicons were released within infectious viral particles only if the cDNA fragment they contain encodes a secretory signal. By using engineered viral replicons to selectively export cDNAs of interest in the culture medium, the methodology reported here efficiently filters genetic information in mammalian cells without the need to select individu

SUBMITTER: Moffatt P 

PROVIDER: S-EPMC140542 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature